Bio-utilization of agricultural residue banana plant shoot through solid state fermentation for production of inulinase using newly isolated Nothophoma anigozanthi JAM

Abstract The present research was carried out with the objectives of isolating fungal strains capable exhibiting inulinase activity from a novel source, then optimizing solid state fermentation for production and characterizing crude enzyme. Production is first reported in this study substrate, banana plant shoot powder, using newly isolated strain Nothophoma anigozanthi JAM through fermentation. root soil white Jamun ( Syzygium cumini ) tree. Optimization by central composite design determining interaction impact influential process on production. A mathematical model developed based statistical analysis recommended optimal conditions 2 g 5 mL moistening media, inoculum volume, 96 h time enhanced (2.578 U/gds). Crude enzyme exhibited an 8.0-fold increase after optimization, comparison under unoptimized conditions. optimum pH (5.0), temperature (60 °C) activity, K m (0.205 mM) V max (0.333 µM/min) were determined biochemical inulin hydrolytic properties further validated TLC, osazone, Seliwanoff tests. Hence, produced can be successfully utilized edible sugar fructose non-edible polysaccharide inulin, agricultural residues such as